Fig. 5.
(A) microRNA (mRNA) levels of muscle ring finger-1 (MuRF1) (CON: n = 8; MV: n = 7; MV + epoxomicin: n = 7). (B) mRNA levels of atrogin-1/Muscle Atrophy F-box (MaFbx) (CON: n = 8; MV: n = 8; MV + epoxomicin: n = 7). (C) Protein levels for MuRF1 (CON: n = 8; MV: n = 7; MV + epoxomicin: n = 8). (D) Protein levels for atrogin-1/MaFbx (n = 8 per group). Representative blots for MuRF1 and atrogin-1/MaFbx protein are shown above the graph. Values are mean percentage change ± SD. *Significantly different versus CON (P < 0.0167). §Significantly different versus CON and MV + epoxomicin (P < 0.0167). Exact P values for all comparisons are listed below the graph. Acutely anesthetized control animals (CON); 12 h mechanically ventilated animals, no treatment (MV); 12 h of MV, treatment with the proteasome inhibitor epoxomicin (MV + epoxomicin).