Effect of different concentrations of tissue plasminogen activator (t-PA) on the lysis index at 30 min after the clotting time (LI30; A), the lysis index at 45 min (LI45; B), the maximum lysis index (ML; C), and lysis onset time (LOT; D), respectively. The LI30, LI45, and ML were determined in the absence (control) or presence of the antifibrinolytic aprotinin (APTEM). Data represent mean ± SD.