Effects of ALX1393 on mechanical allodynia: Mechanical allodynia as assessed by the Plantar Aesthesiometer (Ugo Basile, Comerio, Italy) during application of the glycine transporter 2 inhibitor ALX1393 (Sigma-Aldrich^®, St. Louis, MO) (0.2, 2, 20, or 200 μg/kg per day, dissolved in (2-hydroxypropyl)-β-cyclodextrin) or vehicle. The line graphs show paw-withdrawal thresholds (g) from the injured left (A) and uninjured right paw (B) over time during application. Data are presented as mean ± SEM (n = 6). An asterisk indicates a significant difference compared with vehicle controls (P < 0.05).