Fig. 9.
Effect of valproic acid (VPA) and zinc protoporphyrin IX (ZnPP) on the expression of nuclear levels of nuclear factor (NF)-κB p65 and cytoplasmic levels of inhibitor of NF-κB (IκB)-α in the lung tissues. VPA increased IκB-α level (A) and reduced nuclear NF-κB p65 level (B) in ischemia–reperfusion (I/R)–induced lung injury. ZnPP treatment partially attenuated the protective effect of VPA. TATA and β-actin served as loading controls for nuclear and cytoplasmic proteins, respectively. Representative blots are shown. Data are expressed as mean ± SD (n = 3 per group). ***P < 0.001 compared with the control group; +++P < 0.001 compared with the I/R–vehicle group; #P < 0.05, ##P < 0.01 compared with the I/R + VPA group. +P < 0.05.