Fig. 3.
Deletion of S1PR2 in macrophages improves host defense against intratracheal bacterial infection. (A) Numbers of bacteria recovered in bronchoalveolar lavage fluid (BALF) from various bone marrow (BM) chimeric mice at 4 h after Escherichia coli infection. Data were analyzed by one-way ANOVA with Bonferroni corrections for multiple comparisons and represented by mean ± SD. (B) Survival of BM chimeras as indicated. n = 10 for wild-type (WT, S1pr2+/+) →WT chimeras, and n = 8 for S1pr2−/−→WT chimeras. Data were analyzed by the Mantel–Cox test. (C, D) WT and S1pr2−/− mice were intratracheally treated with control or clondronate liposomes before E. coli challenge. Bacteria in BALF were quantified at 4 h (C), and survival rates were evaluated (D). Data are presented as mean ± SD and were analyzed using one-way ANOVA with Bonferroni corrections (C) or Mantel–Cox test (D). CFU = colony forming units; S1PR2 = sphingosine 1-phosphate receptor 2. *P < 0.05.