Effect of components of prothrombin complex concentrate (PCC) on the peak level of thrombin generation and area under the curve (AUC) of thrombin in the presence of 1 µM dabigatran. Purified prothrombin (FII), prothrombin and FX (FII&X), prothrombin and FX and FIX (FII&X&IX), or 1 U/ml PCC (PCC) were added to platelet-rich plasma assayed in the cell-based CAT^® in the presence of 1 µM dabigatran. The purified coagulation factors were added at levels equal to the levels of these factors present in the PCC. Bars represent the mean (± SD) of three experiments. The black circles represent the mean values of triplicate wells for each of the experiments. Results are expressed as a percent of the control (no additions) in the same experiment. The results in the presence of 1 µM dabigatran, but no added coagulation factors, are indicated as “no reversal.” Data for the AUC are shown in A and peak thrombin in B. *Value significantly different from “no reversal”; #value significantly different from dabigatran + 1 U/ml PCC (PCC) by repeated-measures ANOVA with Tukey multiple comparisons test. Thus, the AUC (A) was significantly greater in the presence of PCC than any of the combinations of added factors. However, the peak thrombin (B) in the presence of dabigatran plus factors II/X/IX was not significantly different from dabigatran plus PCC.