Fig. 5.
(A) Representative Western blot analysis of total protein kinase B (Akt) and phosphorylated Akt (P-Akt) at Ser473 in left ventricular myocardial samples after anesthetic postconditioning with or without hyperglycemia. In each lane, the protein content was 100 μg. β-Actin was used to demonstrate equal protein loading. (B) Graphic presentation of P-Akt quantified by integrating the volume of autoradiograms from three separate experiments. (C) Representative Western blot analysis of total and phosphorylated eNOS (P-eNOS) at Ser1177 in left ventricular myocardial samples after anesthetic postconditioning with or without hyperglycemia. In each lane, the protein content was 100 μg. β-Actin was used to demonstrate equal protein loading. (D) Graphic presentation of P-eNOS expression from the various experimental groups quantified by integrating the volume of autoradiograms from three separate experiments. Values in the graphs are presented as mean ± SD. Diaz = diazoxide; eNOS = endothelial nitric oxide synthase; HG = hyperglycemia; I/R = ischemia–reperfusion; ISO = isoflurane postconditioning. *P < 0.05 compared with the I/R group.