Fig. 4.
Alterations in rostroventral medulla (RVM) modulation after neonatal incision are not limited to the initial injury site. (A) After hind paw incision on postnatal day (P)3, all intensities of electrical stimulation (5–200 μA) of the RVM produce inhibition of reflex excitability when mechanical stimuli were applied to either the ipsilateral previously injured hind paw (P3 incision: ipsilateral, n = 5) or the contralateral uninjured hind paw (P3: contralateral, n = 3) in early adulthood (P40). (B) Incision of the forepaw (P3 forepaw incision, n = 4) or hind paw (P3 hind paw incision, n = 4) resulted in the same pattern of hind limb reflex inhibition across all intensities of RVM stimulation at P40. Bars = mean ± SEM, no significant differences between treatment groups, two-way repeated measures ANOVA with incision group as between-subject and stimulus intensity as repeated within-subject variable followed by Bonferroni post hoc comparisons.