Fig. 5.
Effect of temperature changes on channel properties. (A) Example of Na current induced by 0 mV depolarization at 1Hz frequency from a −100mV holding potential while changing temperature from 15° to 25° for both WT and p.L1612P channels (normalized traces). (B) Temperature decrease reduces mean current density in both WT and p.L1612P ***P < 0.001, n = 21 and 15). (C) The decay is slower at lower temperature (***P < 0.001 LP15° vs. LP25°, #P < 0.05, ###P < 0.001 WT15° vs. WT25°, n = 19 for WT and n = 14 for p.L1612P). (D) Cooling induces a small but significant shift of V1/2 of activation towards more hyperpolarized values (WT: V1/2 from −4.64 ± 4.07 to −2.73 ± 3.48 mV, P = 0.002, n = 20; p.L1612P: V1/2 from −1.24 ± 4.11 to 1.09 ± 2.57 mV, P = 0.002, n = 15). (E) No difference is seen in V1/2 of inactivation between 15° and 25° (WT: V1/2 from −55.52 ± 10.07 to −55.42 ± 9.63 mV, P = 0.96, n = 12; p.L1612P: V1/2 from −32.16 ± 5.45 to −36.19 ± 5.99 mV, P = 0.12, n = 7). (F) At 15° the recovery from inactivation is slower with an increase in t1/2 from 3.18 ± 0.84 to 12.68 ± 3.94 ms for WT (P = 0.004, n = 5) and from 1.48 ± 0.21 to 3.86 ± 0.88 ms for p.L1612P (P = 0.001, n = 6). The recovery of the mutant at 15° comes to the same value as for WT at 25°. LP = p.L1612P; WT = wild type. Stimulation protocols are shown as insets.

Effect of temperature changes on channel properties. (A) Example of Na current induced by 0 mV depolarization at 1Hz frequency from a −100mV holding potential while changing temperature from 15° to 25° for both WT and p.L1612P channels (normalized traces). (B) Temperature decrease reduces mean current density in both WT and p.L1612P ***P < 0.001, n = 21 and 15). (C) The decay is slower at lower temperature (***P < 0.001 LP15° vs. LP25°, #P < 0.05, ###P < 0.001 WT15° vs. WT25°, n = 19 for WT and n = 14 for p.L1612P). (D) Cooling induces a small but significant shift of V1/2 of activation towards more hyperpolarized values (WT: V1/2 from −4.64 ± 4.07 to −2.73 ± 3.48 mV, P = 0.002, n = 20; p.L1612P: V1/2 from −1.24 ± 4.11 to 1.09 ± 2.57 mV, P = 0.002, n = 15). (E) No difference is seen in V1/2 of inactivation between 15° and 25° (WT: V1/2 from −55.52 ± 10.07 to −55.42 ± 9.63 mV, P = 0.96, n = 12; p.L1612P: V1/2 from −32.16 ± 5.45 to −36.19 ± 5.99 mV, P = 0.12, n = 7). (F) At 15° the recovery from inactivation is slower with an increase in t1/2 from 3.18 ± 0.84 to 12.68 ± 3.94 ms for WT (P = 0.004, n = 5) and from 1.48 ± 0.21 to 3.86 ± 0.88 ms for p.L1612P (P = 0.001, n = 6). The recovery of the mutant at 15° comes to the same value as for WT at 25°. LP = p.L1612P; WT = wild type. Stimulation protocols are shown as insets.

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