Fig. 4.
Propofol-treated flies show increased arousal thresholds. The percentage of flies responding to either mild or strong mechanical stimuli were assessed at indicated zeitgeber times for flies fed vehicle (VEH) or two different doses of propofol (PPF) from zeitgeber time 18 to 24. “Mild” and “strong” correspond to the strength of the mechanical stimulus (see Materials and Methods). The percentage of flies reported are a proportion of sleeping flies from VEH (n = 137), 0.5 mM propofol (n = 119), and 1.0 mM propofol-fed (n = 112) total flies tested across six replicate experiments; **P < 0.009; *P < 0.02 (Bonferroni corrected), Kruskal–Wallis test.