Fig. 4.
Local infusion of CX546 into the nucleus accumbens (NAc) relieves mechanical hypersensitivity in paw incision (PI)–treated rats. (A) Schematic showing the timeline of pharmacologic experiments. (B) Intra-NAc infusion of CX546 (400 μM/μl) 4 h and 1 day after PI reduced mechanical hypersensitivity in PI-treated rats. Two-way ANOVA with Bonferroni post-test: saline group, n = 6; CX546 group, n = 8; *P = 0.0248, **P = 0.0012. (C) Intra-NAc infusion of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2, 3-dione (NBQX) blocked the analgesic effects of systemic administration of CX546 (10 mg/kg) in PI-treated rats. Unpaired two-tailed Student’s t test: saline group, n = 8; NBQX group, n = 8; **P = 0.0046. I.P. = intraperitoneal; POD = postoperative day.